A new analytical method for determination of ledipasvir and sofosbuvir in pharmaceutical formulations by HPLC method

  • K. Swathi Department of Pharmaceuticals Analaysis, vikas college of Pharmacy, Krishna District, Andhra Pradesh, India
  • P. Venkateswara Rao Department of Pharmaceuticals Analaysis, vikas college of Pharmacy, Krishna District, Andhra Pradesh, India
  • N. Srinivasa Rao Department of Pharmaceuticals Analaysis, vikas college of Pharmacy, Krishna District, Andhra Pradesh, India
Keywords: Sofosbuvir, Ledipasvir, RP-HPLC.

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Sofosbuvir and Ledipasvir in Tablet dosage form. Chromatogram was run through Std Discovery C8 150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1% OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Sofosbuvir and Ledipasvir were found to be 2.367 min and 3.436 min. %RSD of the Sofosbuvir and Ledipasvir were and found to be 0.6 and 0.5 respectively. %Recovery was obtained as 99.61% and 99.80% for Sofosbuvir and Ledipasvir respectively. LOD, LOQ values obtained from regression equations of Sofosbuvir and Ledipasvir were 0.67, 2.02 and 0.70, 2.12 respectively. Regression equation of Sofosbuvir is y = 4266.x + 7700, and y = 4861.x + 2656.of Ledipasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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Published
09-07-2019
How to Cite
K. Swathi, P. Venkateswara Rao, & N. Srinivasa Rao. (2019). A new analytical method for determination of ledipasvir and sofosbuvir in pharmaceutical formulations by HPLC method . International Journal of Research In Pharmaceutical Chemistry and Analysis, 1(3), 59-67. https://doi.org/10.33974/ijrpca.v1i3.113
Section
Research Article